abstract：:Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...

journal_title：Cell calcium

authors： Takahashi T

更新日期：2005-05-01 00:00:00

abstract：:Recent developments in microscopy and fluorescent indicators now make it possible to monitor the activity and localization of membrane ion channels by imaging Ca(2+) flux through individual channels. Such optical approaches have advantages over electrophysiological single-channel techniques in that they are less invas...

journal_title：Cell calcium

authors： Shuai J,Parker I

更新日期：2005-04-01 00:00:00

abstract：:Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...

journal_title：Cell calcium

authors： Egger M,Porzig H,Niggli E,Schwaller B

更新日期：2005-03-01 00:00:00

abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoform...

journal_title：Cell calcium

authors： Guillemette J,Caron AZ,Regimbald-Dumas Y,Arguin G,Mignery GA,Boulay G,Guillemette G

更新日期：2005-02-01 00:00:00

abstract：:Alternative splicing of the sarco/endoplasmic reticulum (SERCA2) Ca2+ pump transcript generates the two isoforms: SERCA2a in left ventricular myocytes (LVM) and SERCA2b in most tissues. Nuclear protein extracts from left ventricular myocytes can cause a decay of the 3'-region of the SERCA2a. To determine if all the do...

journal_title：Cell calcium

authors： Misquitta CM,Ghosh P,Mwanjewe J,Grover AK

更新日期：2005-01-01 00:00:00

abstract：:We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown ...

journal_title：Cell calcium

authors： Martin VV,Beierlein M,Morgan JL,Rothe A,Gee KR

更新日期：2004-12-01 00:00:00

abstract：:This investigation concentrates on the change in Ca(2+) concentration ([Ca(2+)]) caused by ryanodine in U373 MG cells. This cell type from a human astrocytoma is a unique cellular model because it only expresses the type 3 ryanodine receptor (RyR3), which is generally the least abundant isoform. In the presence of phy...

journal_title：Cell calcium

authors： Galiano M,Gasparre G,Lippe C,Cassano G

更新日期：2004-11-01 00:00:00

abstract：:One of the important events during hypoxia or ischemia in the brain (or other organs for that matter, including the myocardium) is the accumulation of Ca2+ ions intracellularly. Although various studies have shown various sources of and routes for Ca2+ entry and accumulation, it is clear now that it is likely that the...

journal_title：Cell calcium

authors： Yao H,Haddad GG

更新日期：2004-09-01 00:00:00

abstract：:Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...

journal_title：Cell calcium

authors： Peers C,Kemp PJ

更新日期：2004-09-01 00:00:00

abstract：:The regulation of the Ca2+ -induced Ca2+ release (CICR) from intracellular stores is a critical step in the cardiac cycle. The inherent positive feedback of CICR should make it a self-regenerating process. It is accepted that CICR must be governed by some negative control, but its nature is still debated. We explore h...

journal_title：Cell calcium

authors： Zoghbi ME,Copello JA,Villalba-Galea CA,Vélez P,Diaz Sylvester PL,Bolaños P,Marcano A,Fill M,Escobar AL

更新日期：2004-08-01 00:00:00

abstract：:Sarcoplasmic reticulum (SR) is abundant in uterine smooth muscle cells. The functional role of this organelle in the regulation of uterine myocytes is not fully understood. The data available in the literature suggest that SR plays a dual role: as a source of calcium and as a calcium sink shaping calcium transients pr...

journal_title：Cell calcium

authors： Shmygol A,Wray S

更新日期：2004-06-01 00:00:00

abstract：:The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...

journal_title：Cell calcium

authors： Verdonck F,Mubagwa K,Sipido KR

更新日期：2004-06-01 00:00:00

abstract：:We have constructed a stochastic model of the inositol 1,4,5-trisphosphate receptor-Ca2+ channel that is based on quantitative measurements of the channel's properties. It displays the observed dependence of the open probability of the channel with cytosolic [Ca2+] and [IP3] and gives values for the dwell times that a...

journal_title：Cell calcium

authors： Fraiman D,Dawson SP

更新日期：2004-05-01 00:00:00

abstract：:A fluid stream induced by a concentration clamp system evokes in Xenopus oocytes a deformation of the membrane which results in transient chloride currents of high amplitude (stream-evoked inward current, I(i,st)) during calcium-activated chloride current oscillations. The involvement of cytoskeleton elements and of c...

journal_title：Cell calcium

authors： Montag S,Krüger K,Madeja M,Speckmann EJ,Musshoff U

更新日期：2004-04-01 00:00:00

abstract：:Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+]o) raises the intracellular free calcium ([Ca2+]i) and activates differentiation-related genes. Cells lacking the calcium-sensing receptor (CaR) fail to respond to [Ca2+]...

journal_title：Cell calcium

authors： Tu CL,Oda Y,Komuves L,Bikle DD

更新日期：2004-03-01 00:00:00

abstract：:A synthetic peptide (CaMBP) matching amino acids 3614-3643 of the skeletal ryanodine receptor (RyR1) binds to both Ca2+-free calmodulin (CaM) and Ca2+-bound CaM with nanomolar affinity [J. Biol. Chem. 276 (2001) 2069]. We report here that CaMBP increases [3H]ryanodine binding to RyR1 in a dose- and Ca2+-dependent mann...

journal_title：Cell calcium

authors： Zhu X,Ghanta J,Walker JW,Allen PD,Valdivia HH

更新日期：2004-02-01 00:00:00

abstract：:P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...

journal_title：Cell calcium

authors： Fox SC,Behan MW,Heptinstall S

更新日期：2004-01-01 00:00:00

abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...

journal_title：Cell calcium

authors： Putney JW Jr

更新日期：2003-10-01 00:00:00

abstract：:Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Cytoplasmic calcium concentration was measured in primary cultured cells loaded with Fura-2. ET-1 (100 nM) caused c...

journal_title：Cell calcium

authors： Okafor M,Tamiya S,Delamere NA

更新日期：2003-09-01 00:00:00

abstract：:Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...

journal_title：Cell calcium

authors： Hwang SY,Wei J,Westhoff JH,Duncan RS,Ozawa F,Volpe P,Inokuchi K,Koulen P

更新日期：2003-08-01 00:00:00

abstract：:We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...

journal_title：Cell calcium

authors： Burdyga T,Shmygol A,Eisner DA,Wray S

更新日期：2003-07-01 00:00:00

abstract：:Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...

journal_title：Cell calcium

authors： Petersen OH

更新日期：2003-05-01 00:00:00

abstract：:The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...

journal_title：Cell calcium

authors： Trebak M,Vazquez G,Bird GS,Putney JW Jr

更新日期：2003-05-01 00:00:00

abstract：:Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...

journal_title：Cell calcium

authors： Li S,Westwick J,Poll C

更新日期：2003-05-01 00:00:00

abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...

journal_title：Cell calcium

authors： Wuytack F,Raeymaekers L,Missiaen L

更新日期：2002-11-01 00:00:00

abstract：:In the complex signalling interplay that allows extracellular signals to be decoded into activation of apoptotic cell death, Ca(2+) plays a significant role. This is supported not only by evidence linking alterations in Ca(2+) homeostasis to the triggering of apoptotic (and in some cases necrotic) cell death, but also...

journal_title：Cell calcium

authors： Ferrari D,Pinton P,Szabadkai G,Chami M,Campanella M,Pozzan T,Rizzuto R

更新日期：2002-11-01 00:00:00

abstract：:Mammalian homologues of the Drosophila transient receptor potential channel (TRPC) are involved in Ca(2+) entry following agonist stimulation of nonexcitable cells. Seven mammalian TRPCs have been cloned but their mechanisms of activation and/or regulation are still the subject of intense research efforts. It has alre...

journal_title：Cell calcium

authors： Boulay G

更新日期：2002-10-01 00:00:00

abstract：:To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...

journal_title：Cell calcium

authors： Ethier MF,Madison JM

更新日期：2002-07-01 00:00:00

abstract：:The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...

journal_title：Cell calcium

authors： Ziegler A

更新日期：2002-06-01 00:00:00

abstract：:We describe experiments in which the low affinity indicator Oregon Green BAPTA 5N was used to record the spatially resolved changes in [Ca(2+)] from intracellular stores in rat gastric myocytes. Cells were loaded with the membrane permeant form of the indicator and imaged using a confocal scanning laser microscope. In...

journal_title：Cell calcium

authors： White C,McGeown G

更新日期：2002-04-01 00:00:00

abstract：:Rapid formation of high-Ca2+ perimitochondrial cytoplasmic microdomains has been shown to evoke mitochondrial Ca2+ signal and activate mitochondrial dehydrogenases, however, the significance of submicromolar cytoplasmic Ca2+ concentrations in the control of mitochondrial metabolism has not been sufficiently elucidated...

journal_title：Cell calcium

authors： Pitter JG,Maechler P,Wollheim CB,Spät A

更新日期：2002-02-01 00:00:00

abstract：:The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

journal_title：Cell calcium

authors： Tiffert T,Lew VL

更新日期：2001-11-01 00:00:00

abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...

journal_title：Cell calcium

authors： Oishi H,Budel S,Schuster A,Stergiopulos N,Meister JJ,Bény JL

更新日期：2001-10-01 00:00:00

abstract：:The actions of crude venom from Anemesia species of spider were investigated in cultured dorsal root ganglion neurones from neonatal rats and hippocampal slices. Using mass spectrometry (MALDI-TOF MS), 10-12 distinct peptides with masses between about 3 and 10kDa were identified in the crude spider venom. At a concent...

journal_title：Cell calcium

authors： Kalikulov D,Ayar A,Nuritova F,Frenguelli BG,McClelland D,Martin DJ,Davidson I,Scott RH

更新日期：2001-09-01 00:00:00

abstract：:Changes in cytosolic free Ca(2+) concentration ([Ca(2+)]i) were monitored optically in hair cells mechanically isolated from frog semicircular canals using the membrane-impermeant form of the Ca(2+)-selective dye Oregon Green 488 BAPTA-1 (OG, 100 microM). Cells stimulated by depolarization under whole-cell voltage cla...

journal_title：Cell calcium

authors： Rispoli G,Martini M,Rossi ML,Mammano F

更新日期：2001-08-01 00:00:00

abstract：:We have previously demonstrated that bradykinin potentiates prostaglandin E(2)release in human gingival fibroblasts pretreated with interleukin-1 beta (priming). In this study, we demonstrate a potentiating effect of bradykinin on cyclooxygenase-2 mRNA expression in the interleukin-1 beta-primed fibroblasts. Interleuk...

journal_title：Cell calcium

authors： Nakao S,Ogata Y,Modéer T,Segawa M,Furuyama S,Sugiya H

更新日期：2001-06-01 00:00:00

abstract：:Using the low-affinity fluorescent Ca(2+) indicators, Mag-Fura-2 and Mag-Fura Red, we studied light- and InsP(3)-induced Ca(2+) release in permeabilized microvillar photoreceptors of the medicinal leech, Hirudo medicinalis. Two major components of the phosphoinositide signaling pathway, phospholipase-C and the InsP(3)...

journal_title：Cell calcium

authors： Ukhanov K,Mills SJ,Potter BV,Walz B

更新日期：2001-05-01 00:00:00

abstract：:Both theoretical and experimental results are presented for in vivo calibration of the dissociation constant K(Ca)(d)of the calcium-sensitive fluorescent dye Rhod(2)in the perfused mouse heart, using manganese quenching of fluorescence transients. An analytical model is derived, based on the biochemical equilibrium of...

journal_title：Cell calcium

authors： Du C,MacGowan GA,Farkas DL,Koretsky AP

更新日期：2001-04-01 00:00:00

abstract：:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)...

journal_title：Cell calcium

authors： Moerenhout M,Himpens B,Vereecke J

更新日期：2001-02-01 00:00:00

abstract：:An important challenge in the study of Ca2+ signalling is to understand the dynamics of intracellular Ca2+ levels during and after physiological stimulation. While extensive information is available regarding the structural and biophysical properties of Ca2+ channels, pumps and exchangers that control cellular Ca2+ mo...

journal_title：Cell calcium

authors： Friel DD

更新日期：2000-11-01 00:00:00